A Powerful Tool for Monoclonal Antibody Production, Somatic Cell Genetics and Gene Mapping to Chromosomes

PowerPoint Presentation Topics:

• Cell Fusion-- from Cytopathology Observation to Controlled Experimentation
• The Age of Manipulative Biology and the Deliberate Manufacture of Somatic Cell Intra-species and Inter-species Hybrids
• Overview: Fusion Partners (Parental Cells), The Fusion Process from Heterokaryons to Hybrids, The HAT Selection System
• Complementary Parental Cells for TK and HGPRT genes
• Hybrid Cell Line Stability and Random Loss of Genes
• Alternative Fusion Methods
• Use of Hybrids in Cancer Research: Fusion of Normal and Cancer Cells
• Fusion of Antibody Producing Cells and Tumor Cells to Obtain an Immortal Hybridoma Cell Line Secreting One Antibody (Monoclonal Antibody Production)
• Hybrid Cell Lines and Gene Mapping: Fusion Partners, Clonal Lines with Varying Karyotype, Correlation of Loss of Biochemical Trait with Loss of Specific Chromosome
• Cell Reconstruction: The Assembly of Functional Cells with Organelles from Different Cells