Kits
PowerPoint Presentations
Contact
CSK1 Visualization of Normal and Tumor Cells
CSK2 Observation of Heterokaryons Through Cell Fusion
CSK3 Effects of Cytotoxins on Cells
CSK4 Preparation of Human Chromosome Spreads
CSK5 Mouse Chromosome Studies
CSK6 Chromosome Banding
Normal and Cancer Cells In Vitro
Polymerase Chain Reaction (PCR)
Principles of Gel Electrophoresis
Karyotyping
Cell Fusion
Toxicology and Toxicity Testing
Immunology
Chromosome Banding
Immunocytochemistry
In Situ Hybridization
CSPP2 Polymerase Chain Reaction (PCR)

PowerPoint Presentation Topics:

  • The Polymerase Chain Reaction (PCR) Defined
  • The Birth of PCR
  • Molecular Events of the Cycle: Denaturation, Primer Annealing, Primer Extension
  • Final Extension
  • Oligonucleotide Selection: Primer Design and Usage
  • Primer Length and Melting Temperature (Tm)
  • Base Composition
  • Using BLAST for Assessing Primer Design
  • Using the Internet and Software Programs for Primer Design and Usage
  • Properties of Thermostable DNA Polymerases
  • Optimization of the Reaction: A Typical Reaction Mix, Importance of Mg2+ Concentration, Guidelines for Determining Number of Cycles, PCR Additives for Optimization
  • PCR Contamination: Prevention and Control: Variations on the PCR Theme.
PowerPoint Presentations: Each 35 - 45 frame presentation contains text in an outline form, charts, graphs and copies of microscopic observations. The material is supported by a manual for the instructor to follow, giving him or her additional information for each frame of the presentation. The presentations are designed to be covered in a 35 - 40 minute class period.
$39.00 Each
Innovative, inexpensive self-contained kits and PowerPoint presentations for unique hands-on laboratory experiences and instruction in biology, biotechnology and genetics.