PowerPoint Presentation Topics:

• Role in Identification and Localization of Native Nucleic Acids (DNA and RNA) and Foreign Nucleic Acids (infectious organisms, transfected DNA)
• Specimens from Sub-Cellular (chromosomes) to Cellular to Whole Mounts
• Basic Methodology: Hybridization of a Nucleic Acid Target
• Exploitation of Principle of Base Complementarity and Base Pairing Via Hydrogen Bonds
• Analogy with Southern blotting: Specimen Preparation, Probe Design, Stringency Conditions, Visualization of Target-Probe Hybridization
• Probes: DNA or RNA Targets
• Probe Reporters: Radioisotopes, Enzymes, Fluorescent Molecules
• Diverse Applications: Gene Mapping to Chromosomes, Identification of Pathogens, Production and Distribution of mRNA, Detection and Identification of Genetic Diseases and Cancer
• Sample Preparation, Affixing Specimen to Slide, Controls