PowerPoint Presentation Topics:

• The Importance of in vitro Technology: Cell, Tissue and Organ Culture Defined
• Use for Basic and Medical Research: Gene Mapping, Genetic Disease, Growth Factors, The Cell Cycle, Carcinogenesis
• From Biopsy to Culture: Importance of Sterility, Biopsy vs. Whole Animal Embryo as a Cell Source, Tissue Mincing, Tissue Dissociation with Proteolytic Enzymes, The Primary Culture - Survival, Proliferation, and Elimination
• The In Vitro Environment: Physical Environment & Chemical Environment
• Normal Cells: Formation, Attachment, Contact Inhibition, Finite Life Span, and Senescence
• Cancer Cells: In Vivo Behavior, In Vitro Properties, Evaluation of Tumorigenic Potential, Immortal Cells, Chromosome Abnormalities.