PowerPoint: Principles of Gel Electrophoresis
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Topics: General Considerations and Principles: The Electrophoretic Matrix: Properties of Agarose and Acrylamide; An Introduction to the theory of Electrophoresis: Interrelationship of Resistance, Voltage, Current and Power, Electrolysis and pH Effects; Nucleic Acid vs. Protein Separation; Denaturing vs. Non-Denaturing (Native) Electrophoresis; Sample Preparation; Comparison of Buffer Systems for Nucleic Acid Separations; Comparison of Buffer Systems for Protein Separation; Detection of Nucleic Acids in Agarose and Acrylamide Gels; Detection of Nucleic Acids on Blot Transfer Membranes; Detection of Proteins in Acrylamide Gels; Detection of Proteins on Blot Transfer Membranes.