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CSK1 Visualization of Normal and Tumor Cells
CSK2 Observation of Heterokaryons Through Cell Fusion
CSK3 Effects of Cytotoxins on Cells
CSK4 Preparation of Human Chromosome Spreads
CSK5 Mouse Chromosome Studies
CSK6 Chromosome Banding
Normal and Cancer Cells In Vitro
Polymerase Chain Reaction (PCR)
Principles of Gel Electrophoresis
Karyotyping
Cell Fusion
Toxicology and Toxicity Testing
Immunology
Chromosome Banding
Immunocytochemistry
In Situ Hybridization
Kit 6: Chromosome Banding
Kit 6 consists of a set of five G-banded chromosome slides and one PowerPoint presentation describing chromosome banding, methods and applications. The slides provide an opportunity to examine chromosomes banded by the widely used G-banding technique. Hence, the descriptive presentation of the PowerPoint can be enriched by a hands-on microscopy exercise. The cells used as a source of chromosomes is the human cancer cell line, HeLa.

Topics: Definition and Importance of Chromosome Banding in Basic and Clinical Diagnosis; The Ideal Karyotype; From Cell Cultures or Biopsies to Chromosome Spreads for Karyotyping; Solid Stained and Banded Chromosomes Compared; Selected Aspects of Chromosome Nomenclature; Role of Banding in Chromosome Identification; Preparation of Chromosomes for Banding: Avoidance of Condensation, Aged Spreads, Baked Spreads; Trypsin Treatment; Q (Quinicrine), G (Giemsa), R (Reverse), and C (Centromere) Banding: Methods and Outcome; The Paris Conference Standard; Base Composition and Bands; Relaxation to Increase Resolution and Band number; Comparison of Banding Patterns of 350, 800, and 1200 Band Preparations; Analysis of Chromosome Aberrations Including Deletions, Additions, and Translocations; Other Banding Techniques and their Applications.
Also suitable for genetics, cytogenetics, cell biology, and oncology courses.
$99.00 Each Kit (includes companion PowerPoint Presentation)

Because banding requires diligent oversight and care as well as standardized trypsin treatment, we provide for class use stained slides of chromosomes. Instructors can also purchase baked slides of cells which require trypsin-treatment and Giemsa staining. We recommend unstained/untreated chromosome slides be purchased only for small groups of careful students.
5 additional G-Banded chromosome slides - $60
5 unstained baked slides, but not trypsin treated cells - $50
Kit includes
  • 5 G-banded chromosome slides
  • Chromosome Banding PowerPoint Presentation (CSPP8)
  • PowerPoint Instructor's Guide
  • Printed background and procedural information as well as a Glossary of Terms, References, & Further Reading.

Teacher's Preparation

  1. Binocular or monocular microscopes - magnification of 400X to 1000X (oil immersion).

Time Requirements
40-60 minutes.
Innovative, inexpensive self-contained kits and PowerPoint presentations for unique hands-on laboratory experiences and instruction in biology, biotechnology and genetics.